Mouse monoclonal antibody

Antigen/Purification: ExpandCollapse

The antigen is a purified rat Cdk5.

The antibody is protein G purified culture supernatant.

Biological Significance: ExpandCollapse

The neuronal protein kinase, Cdk5 has been implicated in a vast array of normal neuronal functions including regulation of neurotransmitter synthesis (Kansy J et al., 2004), the presynaptic vesicle cycle (Nguyen, C. & Bibb, JA 2003), neurotransmitter receptor trafficking and dopamine neurotransmission (Bibb, JA et al. 1999). At the same time Cdk5 has been implicated in a plethora of neurological and neuropsychiatric disorders including Alzheimer’s, Parkinson’s, Huntington’s, epilepsy, schizophrenia, and drug addiction. Detection of Cdk5 in normal samples as well as tissue undergoing neurodegeneration may advance studies in these areas. Moreover, this antibody may allow more accurate postmortem evaluations of Cdk5 protein expression, and thus serve as a valuable new reagent for neuropathology.

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Hernandez, A., Tan, C., Mettlach, G., Pozo, K., Plattner, F., & Bibb, J. A. (2016). Cdk5 Modulates Long-Term Synaptic Plasticity and Motor Learning in Dorsolateral Striatum. Scientific Reports, 6. PMID: 27443506

Lagace, D. C. et al. Cdk5 is essential for adult hippocampal neurogenesis. Proc Natl Acad Sci USA 105, 18567–18571 (2008).  PMID: 19017796